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Tissue Factor Recombinant antibody

Tissue Factor Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240784H6

Cat no : 83776-4-RR

Print datasheet

Synonyms

F3, Coagulation factor III, CD142, TFA, TF

验证数据展示

  • WB analysis of HepG2 using 83776-4-RR

    WB analysis of HepG2 using 83776-4-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83776-4-RR (Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83776-4-RR

    IHC staining of human stomach cancer using 83776-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83776-4-RR (Tissue Factor antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • IF Staining of A431 using 83776-4-RR

    IF Staining of A431 using 83776-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Tissue Factor antibody (83776-4-RR, Clone: 240784H6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83776-4-RR

    Affinity and Kinetic Characterization of 83776-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83776-4-RR against Human Coagulation Factor III/Tissue Factor were performed. The affinity constant is 0.131 nM.

经过测试的应用

Positive WB detected inHepG2 cells, A431 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83776-4-RR targets Tissue Factor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coagulation factor III (thromboplastin, tissue factor)
Synonyms F3, Coagulation factor III, CD142, TFA, TF
Calculated Molecular Weight33 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberNM_001993.5
Gene Symbol F3
Gene ID (NCBI) 2152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13726-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

实验方案

Product Specific Protocols
WB protocol for Tissue Factor antibody 83776-4-RRDownload protocol
IHC protocol for Tissue Factor antibody 83776-4-RRDownload protocol
IF protocol for Tissue Factor antibody 83776-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols