• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Tissue Factor Recombinant antibody, PBS Only

Tissue Factor Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240784H6

Cat no : 83776-4-PBS

Print datasheet

Synonyms

F3, Coagulation factor III, CD142, TFA, TF

验证数据展示

  • WB analysis of HepG2 using 83776-4-RR (same clone as 83776-4-PBS)

    WB analysis of HepG2 using 83776-4-RR (same clone as 83776-4-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83776-4-RR (Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83776-4-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83776-4-RR (same clone as 83776-4-PBS)

    IHC staining of human stomach cancer using 83776-4-RR (same clone as 83776-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83776-4-RR (Tissue Factor antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83776-4-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83776-4-RR (same clone as 83776-4-PBS)

    IF Staining of A431 using 83776-4-RR (same clone as 83776-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Tissue Factor antibody (83776-4-RR, Clone: 240784H6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83776-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83776-4-RR

    Affinity and Kinetic Characterization of 83776-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83776-4-RR against Human Coagulation Factor III/Tissue Factor were performed. The affinity constant is 0.131 nM.

产品信息

83776-4-PBS targets Tissue Factor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coagulation factor III (thromboplastin, tissue factor)
Synonyms F3, Coagulation factor III, CD142, TFA, TF
Calculated Molecular Weight33 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberNM_001993.5
Gene Symbol F3
Gene ID (NCBI) 2152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13726-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).