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验证数据展示

  • WB analysis of HepG2 using 83776-4-RR

    WB analysis of HepG2 using 83776-4-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83776-4-RR (Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83776-4-RR

    IHC staining of human stomach cancer using 83776-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83776-4-RR (Tissue Factor antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • IF Staining of A431 using 83776-4-RR

    IF Staining of A431 using 83776-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Tissue Factor antibody (83776-4-RR, Clone: 240784H6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83776-4-RR

    Affinity and Kinetic Characterization of 83776-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83776-4-RR against Human Coagulation Factor III/Tissue Factor were performed. The affinity constant is 0.131 nM.

Tissue Factor Recombinant antibody

Tissue Factor Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83776-4-RR

产品说明书

CloneNo. 240784H6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

F3, Coagulation factor III, CD142, TFA, TF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A431 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83776-4-RR targets Tissue Factor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coagulation factor III (thromboplastin, tissue factor)
别名 F3, Coagulation factor III, CD142, TFA, TF
计算分子量33 kDa
观测分子量45 kDa
GenBank蛋白编号NM_001993.5
基因名称 F3
Gene ID (NCBI) 2152
RRIDAB_3671371
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP13726-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

实验方案

Product Specific Protocols
WB protocol for Tissue Factor antibody 83776-4-RRDownload protocol
IHC protocol for Tissue Factor antibody 83776-4-RRDownload protocol
IF protocol for Tissue Factor antibody 83776-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols