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验证数据展示

  • WB analysis using 83782-5-RR

    WB analysis using 83782-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83782-5-RR (ISLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 83782-5-RR

    IHC staining of mouse eye using 83782-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83782-5-RR (ISLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83782-5-RR

    Affinity and Kinetic Characterization of 83782-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83782-5-RR against Human ISLR were performed. The affinity constant is 0.369 nM.

ISLR Recombinant antibody

ISLR Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83782-5-RR

产品说明书

CloneNo. 240727D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

UNQ189/PRO215, Immunoglobulin superfamily containing leucine-rich repeat protein, HsT17563, 240727D4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse retina tissue, NIH/3T3 cells
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83782-5-RR targets ISLR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ISLR fusion protein Ag19446 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 immunoglobulin superfamily containing leucine-rich repeat
别名 UNQ189/PRO215, Immunoglobulin superfamily containing leucine-rich repeat protein, HsT17563, 240727D4
计算分子量 428 aa, 46 kDa
观测分子量44-46 kDa
GenBank蛋白编号BC022478
基因名称 ISLR
Gene ID (NCBI) 3671
RRIDAB_3671374
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO14498
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Immunoglobulin superfamily containing leucine-rich repeat protein(ISLR), is involved in various biological events, such as embryonic development, Gaucher's disease, and replicative senescence of fibroblasts in some organs, including the heart, pancreas, and bone marrow. ISLR, also known as meflin, is a newly discovered marker of mesenchymal stem cells, which is involved in pathological fibrosis and the cancer microenvironment. (PMID: 34713300)

实验方案

Product Specific Protocols
WB protocol for ISLR antibody 83782-5-RRDownload protocol
IHC protocol for ISLR antibody 83782-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols