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ATP2B1 Recombinant antibody, PBS Only (Capture)

ATP2B1 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240848D10

Cat no : 83827-3-PBS

Print datasheet

Synonyms

PMCA1kb, PMCA1, 240848D10

验证数据展示

  • WB analysis of mouse brain using 83827-3-RR (same clone as 83827-3-PBS)

    WB analysis of mouse brain using 83827-3-RR (same clone as 83827-3-PBS)

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 83827-3-RR (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83827-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 83827-3-RR (same clone as 83827-3-PBS)

    IHC staining of mouse small intestine using 83827-3-RR (same clone as 83827-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83827-3-RR (ATP2B1 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83827-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP2B1 antibody included
  • Cytometric bead array standard curve of MP00808-1

    Cytometric bead array standard curve of MP00808-1

    Cytometric bead array standard curve of MP00808-1, ATP2B1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83827-3-PBS. Detection antibody: 83827-2-PBS. Standard: Ag32423. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83827-3-RR

    Affinity and Kinetic Characterization of 83827-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83827-3-RR against Human ATP2B1 were performed. The affinity constant is 10.3 nM.

Planning an IHC experiment? We recommend our IHCeasy ATP2B1 Ready-To-Use IHC Kit. ATP2B1 primary antibody included.

产品信息

83827-3-PBS targets ATP2B1 as part of a matched antibody pair:

MP00808-1: 83827-3-PBS capture and 83827-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATP2B1 fusion protein Ag32423 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, Ca++ transporting, plasma membrane 1
Synonyms PMCA1kb, PMCA1, 240848D10
Calculated Molecular Weight135 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001001323.1
Gene Symbol ATP2B1
Gene ID (NCBI) 490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20020
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).