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ATP2B1 Recombinant antibody

ATP2B1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240848D10

Cat no : 83827-3-RR

Print datasheet

Synonyms

PMCA1kb, PMCA1, 240848D10

验证数据展示

  • WB analysis of mouse brain using 83827-3-RR

    WB analysis of mouse brain using 83827-3-RR

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 83827-3-RR (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 83827-3-RR

    IHC staining of mouse small intestine using 83827-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83827-3-RR (ATP2B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP2B1 antibody included
  • Affinity and Kinetic Characterization of 83827-3-RR

    Affinity and Kinetic Characterization of 83827-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83827-3-RR against Human ATP2B1 were performed. The affinity constant is 10.3 nM.

经过测试的应用

Positive WB detected inunboiled mouse brain tissue
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ATP2B1 Ready-To-Use IHC Kit. ATP2B1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83827-3-RR targets ATP2B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATP2B1 fusion protein Ag32423 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, Ca++ transporting, plasma membrane 1
Synonyms PMCA1kb, PMCA1, 240848D10
Calculated Molecular Weight135 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001001323.1
Gene Symbol ATP2B1
Gene ID (NCBI) 490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20020
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).

实验方案

Product Specific Protocols
WB protocol for ATP2B1 antibody 83827-3-RRDownload protocol
IHC protocol for ATP2B1 antibody 83827-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols