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验证数据展示

  • WB analysis of mouse brain using 83827-3-RR (same clone as 83827-3-PBS)

    WB analysis of mouse brain using 83827-3-RR (same clone as 83827-3-PBS)

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 83827-3-RR (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83827-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 83827-3-RR (same clone as 83827-3-PBS)

    IHC staining of mouse small intestine using 83827-3-RR (same clone as 83827-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83827-3-RR (ATP2B1 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83827-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP2B1 antibody included
  • Cytometric bead array standard curve of MP00808-1

    Cytometric bead array standard curve of MP00808-1

    Cytometric bead array standard curve of MP00808-1, ATP2B1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83827-3-PBS. Detection antibody: 83827-2-PBS. Standard: Ag32423. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83827-3-PBS

    Affinity and Kinetic Characterization of 83827-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83827-3-PBS against Human ATP2B1 was performed. The affinity constant is 4.46 nM.

  • Affinity and Kinetic Characterization of 83827-3-RR

    Affinity and Kinetic Characterization of 83827-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83827-3-RR against Human ATP2B1 were performed. The affinity constant is 10.3 nM.

ATP2B1 Recombinant antibody, PBS Only (Capture)

ATP2B1 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 83827-3-PBS

产品说明书

CloneNo. 240848D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

PMCA1kb, PMCA1, 240848D10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ATP2B1 Ready-To-Use IHC Kit. ATP2B1 primary antibody included.

产品信息

83827-3-PBS targets ATP2B1 as part of a matched antibody pair:

MP00808-1: 83827-3-PBS capture and 83827-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ATP2B1 fusion protein Ag32423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, Ca++ transporting, plasma membrane 1
别名 PMCA1kb, PMCA1, 240848D10
计算分子量135 kDa
观测分子量130 kDa
GenBank蛋白编号NM_001001323.1
基因名称 ATP2B1
Gene ID (NCBI) 490
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20020
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).