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PROSC Recombinant antibody

PROSC Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240998B6

Cat no : 83831-5-RR

Print datasheet

Synonyms

PLPBP, Pyridoxal phosphate-binding protein, Pyridoxal phosphate homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, PLP homeostasis protein

验证数据展示

  • WB analysis using 83831-5-RR

    WB analysis using 83831-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83831-5-RR (PROSC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83831-5-RR

    Affinity and Kinetic Characterization of 83831-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83831-5-RR against Human PROSC were performed. The affinity constant is 2.00 nM.

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, Jurkat cells, L02 cells, mouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83831-5-RR is PROSC Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PROSC fusion protein Ag18369 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name proline synthetase co-transcribed homolog (bacterial)
Synonyms PLPBP, Pyridoxal phosphate-binding protein, Pyridoxal phosphate homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, PLP homeostasis protein
Calculated Molecular Weight 275 aa, 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC012334
Gene Symbol PROSC
Gene ID (NCBI) 11212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO94903
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human Pyridoxal 5′-phosphate-binding protein (PLPBP; previously proline synthase cotranscribed homolog, PROSC) was first described in 1999 and was demonstrated to be ubiquitously expressed in many tissues. PROSC is ubiquitously expressed in human tissues and is highly conserved throughout evolution. PROSC deficiency could therefore markedly increase free cellular PLP levels (PMID: 31825581, 27912044).

实验方案

Product Specific Protocols
WB protocol for PROSC antibody 83831-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols