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BCRP/ABCG2 Recombinant antibody

BCRP/ABCG2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240959A4

Cat no : 83835-1-RR

Print datasheet

Synonyms

ABCG2, BCRP,ABCG2, BCRP, ABCP, ABC15

验证数据展示

  • WB analysis using 83835-1-RR

    WB analysis using 83835-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83835-1-RR (BCRP,ABCG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83835-1-RR

    Affinity and Kinetic Characterization of 83835-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83835-1-RR against Human BCRP,ABCG2 were performed. The affinity constant is 9.85 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, U-251 cells, MCF-7 cells, SGC-7901 cells, HepG2 cells, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83835-1-RR is BCRP/ABCG2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen BCRP/ABCG2 fusion protein Ag25911 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATP-binding cassette, sub-family G (WHITE), member 2
Synonyms ABCG2, BCRP,ABCG2, BCRP, ABCP, ABC15
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 68-72 kDa
GenBank Accession NumberBC021281
Gene Symbol ABCG2
Gene ID (NCBI) 9429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UNQ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.

实验方案

Product Specific Protocols
WB protocol for BCRP/ABCG2 antibody 83835-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols