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验证数据展示

  • WB analysis using 83835-1-RR (same clone as 83835-1-PBS)

    WB analysis using 83835-1-RR (same clone as 83835-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83835-1-RR (BCRP,ABCG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83835-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00805-1

    Cytometric bead array standard curve of MP00805-1

    Cytometric bead array standard curve of MP00805-1, BCRP/ABCG2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83835-1-PBS. Detection antibody: 83835-2-PBS. Standard: Ag25911. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83835-1-PBS

    Affinity and Kinetic Characterization of 83835-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83835-1-PBS against Human BCRP/ABCG2 was performed. The affinity constant is 4.49 nM.

  • Affinity and Kinetic Characterization of 83835-1-RR

    Affinity and Kinetic Characterization of 83835-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83835-1-RR against Human BCRP,ABCG2 were performed. The affinity constant is 9.85 nM.

BCRP/ABCG2 Recombinant antibody, PBS Only (Capture)

BCRP/ABCG2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 83835-1-PBS

产品说明书

CloneNo. 240959A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

ABCG2, BCRP,ABCG2, BCRP, ABCP, ABC15

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83835-1-PBS targets BCRP/ABCG2 as part of a matched antibody pair:

MP00805-1: 83835-1-PBS capture and 83835-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 BCRP/ABCG2 fusion protein Ag25911 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP-binding cassette, sub-family G (WHITE), member 2
别名 ABCG2, BCRP,ABCG2, BCRP, ABCP, ABC15
计算分子量 70 kDa
观测分子量 68-72 kDa
GenBank蛋白编号BC021281
基因名称 ABCG2
Gene ID (NCBI) 9429
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UNQ0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.