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验证数据展示

  • WB analysis of HepG2 using 83838-5-RR

    WB analysis of HepG2 using 83838-5-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83838-5-RR (TFPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83838-5-RR

    FC experiment of HepG2 using 83838-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TFPI Recombinant antibody (83838-5-RR, Clone:240977B1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HUVEC using 83838-5-RR

    FC experiment of HUVEC using 83838-5-RR

    1x10^6 HUVEC cells were surface stained with 0.25 ug TFPI Recombinant antibody (83838-5-RR, Clone:240977B1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • IF Staining of HepG2 using 83838-5-RR

    IF Staining of HepG2 using 83838-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TFPI antibody (83838-5-RR, Clone: 240977B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83838-5-RR

    Affinity and Kinetic Characterization of 83838-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83838-5-RR against Human TFPI were performed. The affinity constant is below 1 pM.

TFPI Recombinant antibody

TFPI Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83838-5-RR

产品说明书

CloneNo. 240977B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

TFI, Lipoprotein-associated coagulation inhibitor, LACI, Extrinsic pathway inhibitor, EPI

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83838-5-RR targets TFPI in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
别名 TFI, Lipoprotein-associated coagulation inhibitor, LACI, Extrinsic pathway inhibitor, EPI
计算分子量35 kDa
观测分子量 40 kDa
GenBank蛋白编号NM_006287.6
基因名称 TFPI
Gene ID (NCBI) 7035
RRIDAB_3671421
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10646-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

实验方案

Product Specific Protocols
WB protocol for TFPI antibody 83838-5-RRDownload protocol
IF protocol for TFPI antibody 83838-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols