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CYFIP2 Recombinant antibody

CYFIP2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240909E3

Cat no : 83845-3-RR

Print datasheet

Synonyms

CYFIP1/2, 240909E3, p53-inducible protein 121, p53 inducible protein 121, KIAA1168

验证数据展示

  • IF Staining of Jurkat using 83845-3-RR

    IF Staining of Jurkat using 83845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CYFIP2 antibody (83845-3-RR, Clone: 240909E3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of Jurkat using 83845-3-RR

    FC experiment of Jurkat using 83845-3-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Cyfip2 Recombinant Antibody (83845-3-RR, Clone:240909E3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CYFIP2 Ready-To-Use IHC Kit. CYFIP2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83845-3-RR targets CYFIP2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CYFIP2 fusion protein Ag8749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytoplasmic FMR1 interacting protein 2
Synonyms CYFIP1/2, 240909E3, p53-inducible protein 121, p53 inducible protein 121, KIAA1168
Calculated Molecular Weight 148 kDa
GenBank Accession NumberBC011762
Gene Symbol CYFIP2
Gene ID (NCBI) 26999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96F07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYFIP1/2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. CYFIP1 contains 1,253 amino acids and shares 88% sequence identity with CYFIP2. CYFIP1 and CYFIP2 are members of a highly conserved protein family and share approximately 99% sequence identity with their mouse orthologs. CYFIP1 has been shown to interact with the small GTPase RAC1, which is implicated in development and maintenance of neuronal structures. Consistent with FMRP and RAC1 localization in dendritic fine structures, CYFIP1/2 are present in synaptosomal extracts. This antibody can recognize both CYFIP1 and CYFIP2.

实验方案

Product Specific Protocols
IF protocol for CYFIP2 antibody 83845-3-RRDownload protocol
FC protocol for CYFIP2 antibody 83845-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols