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验证数据展示

  • WB analysis using 83869-1-RR

    WB analysis using 83869-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83869-1-RR (CUL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 83869-1-RR

    FC experiment of MCF-7 using 83869-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cullin 5 Recombinant antibody (83869-1-RR, Clone:240737A12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Cullin 5 Recombinant antibody

Cullin 5 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83869-1-RR

产品说明书

CloneNo. 240737A12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

CUL5, VACM 1, Cullin-5, CUL-5, CUL 5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, LNCaP cells, NIH/3T3 cells
Positive FC (Intra) detected inMCF-7 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83869-1-RR targets Cullin 5 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 Cullin 5 fusion protein Ag34620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cullin 5
别名 CUL5, VACM 1, Cullin-5, CUL-5, CUL 5
计算分子量 91 kDa
观测分子量80 kDa
GenBank蛋白编号BC063306
基因名称 Cullin 5
Gene ID (NCBI) 8065
RRIDAB_3671455
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ93034
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cullin family proteins are scaffold proteins for the RING finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. In humans, there are eight members of cullins, including CUL1, 2, 3, 4A, 4B, 5, 7, and CUL9 with an evolutionarily conserved cullin homology domain at the C-terminus, responsible for their interaction with the RING protein, assembling 8 cullin-based RING dependent E3 ubiquitin ligases to target thousands of substrates for ubiquitination and degradation (PMID: 32334051). Cullin 5 is the key scaffolding protein of Cullin RING ligase-5 (CRL-5). Cullin 5 plays positive roles in regulating cell growth, proliferation and physiological and other processes in the human body (PMID: 32194910). Cullin 5 has been detected in the form of double bands in the results of Western blot, which might be caused by neddylation (PMID: 36449095; 31294695).

实验方案

Product Specific Protocols
WB protocol for Cullin 5 antibody 83869-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols