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Cullin 5 Recombinant antibody, PBS Only

Cullin 5 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240737A12

Cat no : 83869-1-PBS

Print datasheet

Synonyms

CUL5, VACM 1, Cullin-5, CUL-5, CUL 5

验证数据展示

  • WB analysis using 83869-1-RR (same clone as 83869-1-PBS)

    WB analysis using 83869-1-RR (same clone as 83869-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83869-1-RR (CUL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83869-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83869-1-RR (same clone as 83869-1-PBS)

    FC experiment of MCF-7 using 83869-1-RR (same clone as 83869-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cullin 5 Recombinant antibody (83869-1-RR, Clone:240737A12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83869-1-PBS in a different storage buffer formulation.

产品信息

83869-1-PBS targets Cullin 5 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen Cullin 5 fusion protein Ag34620 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cullin 5
Synonyms CUL5, VACM 1, Cullin-5, CUL-5, CUL 5
Calculated Molecular Weight 91 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC063306
Gene Symbol Cullin 5
Gene ID (NCBI) 8065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ93034
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cullin family proteins are scaffold proteins for the RING finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. In humans, there are eight members of cullins, including CUL1, 2, 3, 4A, 4B, 5, 7, and CUL9 with an evolutionarily conserved cullin homology domain at the C-terminus, responsible for their interaction with the RING protein, assembling 8 cullin-based RING dependent E3 ubiquitin ligases to target thousands of substrates for ubiquitination and degradation (PMID: 32334051). Cullin 5 is the key scaffolding protein of Cullin RING ligase-5 (CRL-5). Cullin 5 plays positive roles in regulating cell growth, proliferation and physiological and other processes in the human body (PMID: 32194910). Cullin 5 has been detected in the form of double bands in the results of Western blot, which might be caused by neddylation (PMID: 36449095; 31294695).