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验证数据展示

  • WB analysis of mouse brown adipose using 83870-1-RR

    WB analysis of mouse brown adipose using 83870-1-RR

    mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 83870-1-RR (UCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 83870-1-RR

    IHC staining of rat brain using 83870-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83870-1-RR (UCP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brown adipose using 83870-1-RR

    IF Staining of mouse brown adipose using 83870-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using UCP1 antibody (83870-1-RR, Clone: 240782F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83870-1-RR

    Affinity and Kinetic Characterization of 83870-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83870-1-RR against Human UCP1 were performed. The affinity constant is 64.6 nM.

UCP1 Recombinant antibody

UCP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83870-1-RR

产品说明书

CloneNo. 240782F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

UCP 1, UCP, Thermogenin, SLC25A7, 240782F3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brown adipose tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brown adipose tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83870-1-RR targets UCP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 UCP1 fusion protein Ag20388 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 uncoupling protein 1 (mitochondrial, proton carrier)
别名 UCP 1, UCP, Thermogenin, SLC25A7, 240782F3
计算分子量 307 aa, 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC098168
基因名称 UCP1
Gene ID (NCBI) 7350
RRIDAB_3671456
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP25874
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

实验方案

Product Specific Protocols
WB protocol for UCP1 antibody 83870-1-RRDownload protocol
IHC protocol for UCP1 antibody 83870-1-RRDownload protocol
IF protocol for UCP1 antibody 83870-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols