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Host
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis of mouse brown adipose using 83870-1-RR (same clone as 83870-1-PBS)

    WB analysis of mouse brown adipose using 83870-1-RR (same clone as 83870-1-PBS)

    mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 83870-1-RR (UCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 83870-1-RR (same clone as 83870-1-PBS)

    IHC staining of rat brain using 83870-1-RR (same clone as 83870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83870-1-RR (UCP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brown adipose using 83870-1-RR (same clone as 83870-1-PBS)

    IF Staining of mouse brown adipose using 83870-1-RR (same clone as 83870-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using UCP1 antibody (83870-1-RR, Clone: 240782F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83870-1-RR

    Affinity and Kinetic Characterization of 83870-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83870-1-RR against Human UCP1 were performed. The affinity constant is 64.6 nM.

UCP1 Recombinant antibody, PBS Only

UCP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83870-1-PBS

Print datasheet

CloneNo. 240782F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

UCP 1, UCP, Thermogenin, SLC25A7, 240782F3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83870-1-PBS targets UCP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen UCP1 fusion protein Ag20388 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name uncoupling protein 1 (mitochondrial, proton carrier)
Synonyms UCP 1, UCP, Thermogenin, SLC25A7, 240782F3
Calculated Molecular Weight 307 aa, 33 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC098168
Gene Symbol UCP1
Gene ID (NCBI) 7350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP25874
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.