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NCAM1/CD56 Recombinant antibody

NCAM1/CD56 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240936B10

Cat no : 83883-5-RR

Print datasheet

Synonyms

Ncam1, CD56, 240936B10, NCAM 1, Ncam

验证数据展示

  • IF Staining of Neuro-2a using 83883-5-RR

    IF Staining of Neuro-2a using 83883-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of Neuro-2a using 83883-5-RR

    IF Staining of Neuro-2a using 83883-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83883-5-RR

    Affinity and Kinetic Characterization of 83883-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83883-5-RR against Mouse Ncam1 were performed. The affinity constant is 0.184 nM.

经过测试的应用

Positive IF/ICC detected inNeuro-2a cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83883-5-RR is NCAM1/CD56 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neural cell adhesion molecule 1
Synonyms Ncam1, CD56, 240936B10, NCAM 1, Ncam
Calculated Molecular Weight119 kDa
Observed Molecular Weight120 kDa, 140 kDa, 180 kDa
Gene Symbol Ncam1
Gene ID (NCBI) 17967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13595-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).

实验方案

Product Specific Protocols
IF protocol for NCAM1/CD56 antibody 83883-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols