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Mouse Tissue Factor Recombinant antibody, PBS Only (Capture/Detector)

Tissue Factor Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240875H3

Cat no : 83887-4-PBS

Print datasheet

Synonyms

F3, Coagulation factor III, CD142, TFA, TF

验证数据展示

  • WB analysis using 83887-4-RR (same clone as 83887-4-PBS)

    WB analysis using 83887-4-RR (same clone as 83887-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83887-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00859-1

    Sandwich ELISA standard curve of MP00859-1

    Sandwich ELISA standard curve of MP00859-1, Mouse Coagulation Factor III/Tissue Factor Recombinant Matched Antibody Pair - PBS only. 83887-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1102. 83887-4-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

  • Sample test of MP00859-1

    Sample test of MP00859-1

    Urine of mice was measured. The Coagulation Factor III/Tissue Factor concentration of detected samples was determined to be 244.1 pg/mL.

  • Sample test of MP00859-1

    Sample test of MP00859-1

    The mean Coagulation Factor III/Tissue Factor concentration was determined to be 18.4 ng/mL in mouse brain cell extract based on a 6.0 mg/mL extract load, 13.1 ng/mL in C2C12 cell extract based on a 3.6 mg/mL extract load and 2.7 ng/mL in NIH/3T3 cell extract based on a 10.6 mg/mL extract load.

  • Sample test of MP00859-1

    Sample test of MP00859-1

    Serum of sixteen mice was measured. The Coagulation Factor III/Tissue Factor concentration of detected samples was determined to be 86.6 pg/mL with a range of 63.0-105.0 pg/mL.

  • Cytometric bead array standard curve of MP00859-1

    Cytometric bead array standard curve of MP00859-1

    Cytometric bead array standard curve of MP00859-1, MOUSE Coagulation Factor III/Tissue Factor Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83887-1-PBS. Detection antibody: 83887-4-PBS. Standard: Eg1102. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00859-2

    Cytometric bead array standard curve of MP00859-2

    Cytometric bead array standard curve of MP00859-2, MOUSE Coagulation Factor III/Tissue Factor Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83887-3-PBS. Detection antibody: 83887-4-PBS. Standard: Eg1102. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP00859-3

    Cytometric bead array standard curve of MP00859-3

    Cytometric bead array standard curve of MP00859-3, MOUSE Coagulation Factor III/Tissue Factor Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83887-4-PBS. Detection antibody: 83887-2-PBS. Standard: Eg1102. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83887-4-RR

    Affinity and Kinetic Characterization of 83887-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83887-4-RR against Mouse Tissue Factor were performed. The affinity constant is 0.85 nM.

产品信息

83887-4-PBS targets Tissue Factor as part of a matched antibody pair:

MP00859-1: 83887-1-PBS capture and 83887-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00859-2: 83887-3-PBS capture and 83887-4-PBS detection (validated in Cytometric bead array)

MP00859-3: 83887-4-PBS capture and 83887-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coagulation factor III
Synonyms F3, Coagulation factor III, CD142, TFA, TF
Calculated Molecular Weight33kDa
Observed Molecular Weight48-53 kDa
Gene Symbol Tissue Factor
Gene ID (NCBI) 14066
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20352
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108). This antibody is specific for mouse Tissue Factor.