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验证数据展示

  • WB analysis of A431 using 83898-1-RR (same clone as 83898-1-PBS)

    WB analysis of A431 using 83898-1-RR (same clone as 83898-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83898-1-PBS in a different storage buffer formulation.

  • WB analysis using 83898-1-RR (same clone as 83898-1-PBS)

    WB analysis using 83898-1-RR (same clone as 83898-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83898-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00839-4

    Sandwich ELISA standard curve of MP00839-4

    Sandwich ELISA standard curve of MP00839-4, Human PLEC/Plectin Recombinant Matched Antibody Pair - PBS only. 83898-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag29418. 83898-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

  • Sample test of MP00839-4

    Sample test of MP00839-4

    U2OS cell lysate, NIH/3T3 cell lysate and C6 cell lysate were measured. The human PLEC/Plectin concentration was determined to be 212.14 ng/mg total protein in U2OS cell lysate, 3,895.97 ng/mg total protein in NIH/3T3 cell lysate and 609.19 ng/mg total protein in C6 cell lysate.

  • IF Staining of A431 using 83898-1-RR (same clone as 83898-1-PBS)

    IF Staining of A431 using 83898-1-RR (same clone as 83898-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLEC antibody (83898-1-RR, Clone: 240891A10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83898-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00839-2

    Cytometric bead array standard curve of MP00839-2

    Cytometric bead array standard curve of MP00839-2, PLEC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83898-1-PBS. Detection antibody: 83898-2-PBS. Standard: Ag29418. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP00839-3

    Cytometric bead array standard curve of MP00839-3

    Cytometric bead array standard curve of MP00839-3, PLEC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83898-1-PBS. Detection antibody: 83898-3-PBS. Standard: Ag29418. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83898-1-PBS

    Affinity and Kinetic Characterization of 83898-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83898-1-PBS against Human PLEC was performed. The affinity constant is 0.164 nM.

  • Affinity and Kinetic Characterization of 83898-1-RR

    Affinity and Kinetic Characterization of 83898-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83898-1-RR against Human PLEC were performed. The affinity constant is 52.7 pM.

PLEC Recombinant antibody, PBS Only (Capture/Detector)

PLEC Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83898-1-PBS

产品说明书

CloneNo. 240891A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PLEC Ready-To-Use IHC Kit. PLEC primary antibody included.

产品信息

83898-1-PBS targets PLEC as part of a matched antibody pair:

MP00839-2: 83898-1-PBS capture and 83898-2-PBS detection (validated in Cytometric bead array)

MP00839-3: 83898-1-PBS capture and 83898-3-PBS detection (validated in Cytometric bead array)

MP00839-4: 83898-4-PBS capture and 83898-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 PLEC fusion protein Ag29418 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 plectin 1, intermediate filament binding protein 500kDa
别名 PLEC1, Plectin, PLTN, Plectin 1, PLEC1b
计算分子量 532 kDa
观测分子量 510 kDa
GenBank蛋白编号NM_201380
基因名称 Plectin
Gene ID (NCBI) 5339
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15149
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)