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KIAA1586 Recombinant antibody

KIAA1586 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240972F10

Cat no : 83900-7-RR

Print datasheet

Synonyms

240972F10

验证数据展示

  • WB analysis using 83900-7-RR

    WB analysis using 83900-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83900-7-RR (KIAA1586 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83900-7-RR

    Affinity and Kinetic Characterization of 83900-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83900-7-RR against Human KIAA1586 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83900-7-RR targets KIAA1586 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen KIAA1586 fusion protein Ag36038 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KIAA1586
Synonyms 240972F10
Calculated Molecular Weight 787 aa, 90 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC041125
Gene Symbol KIAA1586
Gene ID (NCBI) 57691
RRIDAB_3671481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9HCI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1586, close to ZNF451, encodes a protein with similar N-terminal tandem SIMs, which has also shown elongase activity. ZNF451 is an E3 SUMO-protein ligase that facilitates UBE2I/UBC9-mediated SUMO2 modification of target proteins (PMID: 26524493, PMID: 35887358).

实验方案

Product Specific Protocols
WB protocol for KIAA1586 antibody 83900-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols