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PYROXD2 Recombinant antibody

PYROXD2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240984A12

Cat no : 83954-1-RR

Print datasheet

Synonyms

C10orf33, YueF, FP3420, 240984A12

验证数据展示

  • WB analysis using 83954-1-RR

    WB analysis using 83954-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83954-1-RR (PYROXD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83954-1-RR

    WB analysis using 83954-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83954-1-RR (PYROXD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83954-1-RR

    Affinity and Kinetic Characterization of 83954-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83954-1-RR against Human PYROXD2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293T cells, LNCaP cells, U-251 cells, A375 cells, L02 cells, Raji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83954-1-RR is PYROXD2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen PYROXD2 fusion protein Ag14701 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 10 open reading frame 33
Synonyms C10orf33, YueF, FP3420, 240984A12
Calculated Molecular Weight 581 aa, 63 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberBC006131
Gene Symbol PYROXD2
Gene ID (NCBI) 84795
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N2H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pyridine Nucleotide-Disulfide Oxidoreductase Domain 2 (PYROXD2, previously called YueF, C10orf33) is a mitochondrial inner membrane/matrix-residing protein and is reported to regulate mitochondrial function. PYROXD2 is highly expressed in the cytoplasm of normal cells and tissues but is expressed at lower levels in corresponding cancer cells, including liver, lung and renal cell carcinoma and bladder cancer cells (PMID: 29048625, PMID: 35606887).

实验方案

Product Specific Protocols
WB protocol for PYROXD2 antibody 83954-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols