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  • Featured Product
  • KD/KO Validated

PEA15 Recombinant antibody, PBS Only (Capture)

PEA15 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241044G8

Cat no : 83958-2-PBS

Print datasheet

Synonyms

MAT1H, MAT1, HUMMAT1H, HMAT1, Astrocytic phosphoprotein PEA-15

验证数据展示

  • WB analysis using 83958-2-RR (same clone as 83958-2-PBS)

    WB analysis using 83958-2-RR (same clone as 83958-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83958-2-RR (PEA15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83958-2-PBS in a different storage buffer formulation.

  • WB analysis of COLO 320 using 83958-2-RR (same clone as 83958-2-PBS)

    WB analysis of COLO 320 using 83958-2-RR (same clone as 83958-2-PBS)

    WB result of PEA15 antibody (83958-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEA15 transfected COLO 320 cells. This data was developed using the same antibody clone with 83958-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00916-1

    Cytometric bead array standard curve of MP00916-1

    Cytometric bead array standard curve of MP00916-1, PEA15 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83958-2-PBS. Detection antibody: 83958-1-PBS. Standard: Ag13782. Range: 3.125-200 ng/mL.

产品信息

83958-2-PBS targets PEA15 as part of a matched antibody pair:

MP00916-1: 83958-2-PBS capture and 83958-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PEA15 fusion protein Ag13782 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphoprotein enriched in astrocytes 15
Synonyms MAT1H, MAT1, HUMMAT1H, HMAT1, Astrocytic phosphoprotein PEA-15
Calculated Molecular Weight 130 aa, 15 kDa
Observed Molecular Weight 13-15 kDa
GenBank Accession NumberBC002426
Gene Symbol PEA15
Gene ID (NCBI) 8682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15121
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phosphoprotein Enriched in Astrocytes 15 kDa (PEA15), also known as PED-15, is a small, death effector-domain (DED)-containing protein that was recently demonstrated to inhibit tumor necrosis factor-α-induced apoptosis and to reverse the inhibition of integrin activation due to H-Ras. PEA15 is a 15 kDa protein that is highly expressed in the nervous system with particularly high levels in astrocytes and neurons of the hippocampus. PEA15 is a cytoplasmic protein that sits at an important junction in intracellular signalling and can regulate diverse cellular processes, such as proliferation and apoptosis, dependent upon stimulation.