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Adiponectin Recombinant antibody

Adiponectin Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241082E1

Cat no : 83961-3-RR

Print datasheet

Synonyms

ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein

验证数据展示

  • IHC staining of mouse brown adipose using 83961-3-RR

    IHC staining of mouse brown adipose using 83961-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83961-3-RR (ADIPOQ antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Adiponectin antibody included
  • IHC staining of mouse brown adipose using 83961-3-RR

    IHC staining of mouse brown adipose using 83961-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83961-3-RR (ADIPOQ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Adiponectin antibody included
  • IF Staining of 3T3-L1 using 83961-3-RR

    IF Staining of 3T3-L1 using 83961-3-RR

    Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using ADIPOQ antibody (83961-3-RR, Clone: 241082E1 ) at dilution of 1:0 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83961-3-RR

    Affinity and Kinetic Characterization of 83961-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83961-3-RR against Human ADIPOQ were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive IHC detected inmouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in3T3-L1 cells
Planning an IHC experiment? We recommend our IHCeasy Adiponectin Ready-To-Use IHC Kit. Adiponectin primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83961-3-RR is Adiponectin Fusion Protein expressed in E. coli.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Adiponectin fusion protein Ag16304 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name adiponectin, C1Q and collagen domain containing
Synonyms ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein
Calculated Molecular Weight 244 aa, 26 kDa
GenBank Accession NumberBC096308
Gene Symbol Adiponectin
Gene ID (NCBI) 9370
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15848
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

实验方案

Product Specific Protocols
IHC protocol for Adiponectin antibody 83961-3-RRDownload protocol
IF protocol for Adiponectin antibody 83961-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols