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验证数据展示

  • IF Staining of HepG2 using 83973-4-RR

    IF Staining of HepG2 using 83973-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGG1 antibody (83973-4-RR, Clone: 240940D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83973-4-RR

    FC experiment of HeLa using 83973-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ogg1 Recombinant Antibody (83973-4-RR, Clone:240940D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83973-4-RR

    Affinity and Kinetic Characterization of 83973-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83973-4-RR against Human OGG1 were performed. The affinity constant is below 1 pM.

OGG1 Recombinant antibody

OGG1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83973-4-RR

产品说明书

CloneNo. 240940D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Ogg 1, N-glycosylase/DNA lyase, DNA-(apurinic or apyrimidinic site) lyase, 8-oxoguanine DNA glycosylase, 8 oxoguanine DNA glycosylase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83973-4-RR targets OGG1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 OGG1 fusion protein Ag7204 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 8-oxoguanine DNA glycosylase
别名 Ogg 1, N-glycosylase/DNA lyase, DNA-(apurinic or apyrimidinic site) lyase, 8-oxoguanine DNA glycosylase, 8 oxoguanine DNA glycosylase
计算分子量 22 kDa, 36-40 kDa, 45-57 kDa
GenBank蛋白编号BC000657
基因名称 OGG1
Gene ID (NCBI) 4968
RRIDAB_3671548
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15527
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa).

实验方案

Product Specific Protocols
IF protocol for OGG1 antibody 83973-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols