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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • IF Staining of HepG2 using 83973-4-RR (same clone as 83973-4-PBS)

    IF Staining of HepG2 using 83973-4-RR (same clone as 83973-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGG1 antibody (83973-4-RR, Clone: 240940D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83973-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83973-4-RR (same clone as 83973-4-PBS)

    FC experiment of HeLa using 83973-4-RR (same clone as 83973-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ogg1 Recombinant Antibody (83973-4-RR, Clone:240940D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83973-4-PBS in a different storage buffer formulation.

OGG1 Recombinant antibody, PBS Only

OGG1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83973-4-PBS
CloneNo. 240940D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Ogg 1, N-glycosylase/DNA lyase, DNA-(apurinic or apyrimidinic site) lyase, 8-oxoguanine DNA glycosylase, 8 oxoguanine DNA glycosylase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83973-4-PBS targets OGG1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen OGG1 fusion protein Ag7204 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 8-oxoguanine DNA glycosylase
Synonyms Ogg 1, N-glycosylase/DNA lyase, DNA-(apurinic or apyrimidinic site) lyase, 8-oxoguanine DNA glycosylase, 8 oxoguanine DNA glycosylase
Calculated Molecular Weight 22 kDa, 36-40 kDa, 45-57 kDa
GenBank Accession NumberBC000657
Gene Symbol OGG1
Gene ID (NCBI) 4968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15527
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa).