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验证数据展示

  • FC experiment of HeLa using 83974-2-RR

    FC experiment of HeLa using 83974-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-2-RR, Clone:240927A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • WB analysis using 83974-2-RR

    WB analysis using 83974-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83974-2-RR (RNGTT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83974-2-RR

    Affinity and Kinetic Characterization of 83974-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83974-2-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

RNGTT Recombinant antibody

RNGTT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83974-2-RR

产品说明书

CloneNo. 240927A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, K-562 cells, HEK-293T cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83974-2-RR targets RNGTT in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 RNGTT fusion protein Ag3105 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA guanylyltransferase and 5'-phosphatase
别名 hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A
计算分子量 597 aa, 69 kDa
观测分子量62-68 kDa
GenBank蛋白编号BC019954
基因名称 RNGTT
Gene ID (NCBI) 8732
RRIDAB_3671549
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60942
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for RNGTT antibody 83974-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols