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验证数据展示

  • FC experiment of HeLa using 83974-2-RR (same clone as 83974-2-PBS)

    FC experiment of HeLa using 83974-2-RR (same clone as 83974-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-2-RR, Clone:240927A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83974-2-PBS in a different storage buffer formulation.

  • WB analysis using 83974-2-RR (same clone as 83974-2-PBS)

    WB analysis using 83974-2-RR (same clone as 83974-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83974-2-RR (RNGTT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83974-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00899-2

    Cytometric bead array standard curve of MP00899-2

    Cytometric bead array standard curve of MP00899-2, RNGTT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83974-2-PBS. Detection antibody: 83974-3-PBS. Standard: Ag3105. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83974-2-RR

    Affinity and Kinetic Characterization of 83974-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83974-2-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

RNGTT Recombinant antibody, PBS Only (Capture)

RNGTT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83974-2-PBS

产品说明书

CloneNo. 240927A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83974-2-PBS targets RNGTT as part of a matched antibody pair:

MP00899-2: 83974-2-PBS capture and 83974-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 RNGTT fusion protein Ag3105 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA guanylyltransferase and 5'-phosphatase
别名 hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A
计算分子量 597 aa, 69 kDa
观测分子量62-68 kDa
GenBank蛋白编号BC019954
基因名称 RNGTT
Gene ID (NCBI) 8732
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60942
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.