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RNGTT Recombinant antibody, PBS Only (Capture)

RNGTT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240927A10

Cat no : 83974-2-PBS

Print datasheet

Synonyms

hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A

验证数据展示

  • FC experiment of HeLa using 83974-2-RR (same clone as 83974-2-PBS)

    FC experiment of HeLa using 83974-2-RR (same clone as 83974-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-2-RR, Clone:240927A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83974-2-PBS in a different storage buffer formulation.

  • WB analysis using 83974-2-RR (same clone as 83974-2-PBS)

    WB analysis using 83974-2-RR (same clone as 83974-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83974-2-RR (RNGTT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83974-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00899-2

    Cytometric bead array standard curve of MP00899-2

    Cytometric bead array standard curve of MP00899-2, RNGTT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83974-2-PBS. Detection antibody: 83974-3-PBS. Standard: Ag3105. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83974-2-RR

    Affinity and Kinetic Characterization of 83974-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83974-2-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

产品信息

83974-2-PBS targets RNGTT as part of a matched antibody pair:

MP00899-2: 83974-2-PBS capture and 83974-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RNGTT fusion protein Ag3105 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RNA guanylyltransferase and 5'-phosphatase
Synonyms hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A
Calculated Molecular Weight 597 aa, 69 kDa
Observed Molecular Weight62-68 kDa
GenBank Accession NumberBC019954
Gene Symbol RNGTT
Gene ID (NCBI) 8732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60942
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.