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RNGTT Recombinant antibody

RNGTT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240927A10

Cat no : 83974-2-RR

Print datasheet

Synonyms

hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A

验证数据展示

  • FC experiment of HeLa using 83974-2-RR

    FC experiment of HeLa using 83974-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-2-RR, Clone:240927A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • WB analysis using 83974-2-RR

    WB analysis using 83974-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83974-2-RR (RNGTT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83974-2-RR

    Affinity and Kinetic Characterization of 83974-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83974-2-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, K-562 cells, HEK-293T cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83974-2-RR targets RNGTT in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen RNGTT fusion protein Ag3105 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RNA guanylyltransferase and 5'-phosphatase
Synonyms hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A
Calculated Molecular Weight 597 aa, 69 kDa
Observed Molecular Weight62-68 kDa
GenBank Accession NumberBC019954
Gene Symbol RNGTT
Gene ID (NCBI) 8732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for RNGTT antibody 83974-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols