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验证数据展示

  • IF Staining of HepG2 using 83987-2-RR

    IF Staining of HepG2 using 83987-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFE2L1 antibody (83987-2-RR, Clone: 241089D1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83987-2-RR

    Affinity and Kinetic Characterization of 83987-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83987-2-RR against Human NFE2L1 were performed. The affinity constant is 0.988 nM.

NFE2L1 Recombinant antibody

NFE2L1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 83987-2-RR

产品说明书

CloneNo. 241089D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

NF-E2-related factor 1, Locus control region-factor 1, LCR-F1, HBZ17, Endoplasmic reticulum membrane sensor NFE2L1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83987-2-RR targets NFE2L1 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 NFE2L1 fusion protein Ag0525 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear factor (erythroid-derived 2)-like 1
别名 NF-E2-related factor 1, Locus control region-factor 1, LCR-F1, HBZ17, Endoplasmic reticulum membrane sensor NFE2L1
计算分子量 772 aa, 85 kDa
GenBank蛋白编号BC010623
基因名称 NFE2L1
Gene ID (NCBI) 4779
RRIDAB_3671562
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14494
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for NFE2L1 antibody 83987-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols