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验证数据展示

  • WB analysis using 84023-1-RR (same clone as 84023-1-PBS)

    WB analysis using 84023-1-RR (same clone as 84023-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84023-1-RR (CSF2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84023-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84023-1-RR (same clone as 84023-1-PBS)

    IHC staining of human placenta using 84023-1-RR (same clone as 84023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84023-1-RR (CSF2RA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84023-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84023-1-RR (same clone as 84023-1-PBS)

    IHC staining of human placenta using 84023-1-RR (same clone as 84023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84023-1-RR (CSF2RA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84023-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 84023-1-RR (same clone as 84023-1-PBS)

    IF Staining of THP-1 using 84023-1-RR (same clone as 84023-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CSF2RA antibody (84023-1-RR, Clone: 241266A9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84023-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00953-1

    Sandwich ELISA standard curve of MP00953-1

    Sandwich ELISA standard curve of MP00953-1, Human CSF2RA/CD116 Recombinant Matched Antibody Pair - PBS only. 98066-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02335. 84023-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00953-1

    Sample test of MP00953-1

    The mean CSF2RA/CD116 concentration was determined to be 84.1 ng/mL in THP-1 cell extract based on a 4.0 mg/mL extract load and 59.0 ng/mL in HL-60 cell extract based on a 5.6 mg/mL extract load.

  • Sample test of MP00953-1

    Sample test of MP00953-1

    Serum of six individual healthy human donors was measured. The CSF2RA/CD116 concentration of detected samples was determined to be 2.0 ng/mL with a range of 0.9-4.7 ng/mL.

  • Cytometric bead array standard curve of MP00953-1

    Cytometric bead array standard curve of MP00953-1

    Cytometric bead array standard curve of MP00953-1, CSF2RA/CD116 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98066-1-PBS. Detection antibody: 84023-1-PBS. Standard: RP02335. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84023-1-RR

    Affinity and Kinetic Characterization of 84023-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84023-1-RR against Human CSF2RA/CD116 were performed. The affinity constant is 1.05 nM.

CSF2RA/CD116 Recombinant antibody, PBS Only (Detector)

CSF2RA/CD116 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84023-1-PBS

产品说明书

CloneNo. 241266A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

CD116, 241266A9, GMCSFR, GM CSF R alpha, CSF2RA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84023-1-PBS targets CSF2RA/CD116 as part of a matched antibody pair:

MP00953-1: 98066-1-PBS capture and 84023-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
别名 CD116, 241266A9, GMCSFR, GM CSF R alpha, CSF2RA
计算分子量 46 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC002635
基因名称 CD116
Gene ID (NCBI) 1438
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15509
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.