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L1CAM Recombinant antibody

L1CAM Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240978F1

Cat no : 84034-4-RR

Print datasheet

Synonyms

L1 cell adhesion molecule, HSAS1, HSAS, CD171, CAML1

验证数据展示

  • WB analysis using 84034-4-RR

    WB analysis using 84034-4-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 84034-4-RR (L1CAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84034-4-RR

    Affinity and Kinetic Characterization of 84034-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84034-4-RR against Human L1CAM were performed. The affinity constant is 0.255 nM.

经过测试的应用

Positive WB detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84034-4-RR is L1CAM Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name L1 cell adhesion molecule
Synonyms L1 cell adhesion molecule, HSAS1, HSAS, CD171, CAML1
Calculated Molecular Weight 1257 aa, 140 kDa
Observed Molecular Weight245 kDa
GenBank Accession NumberBC126229
Gene Symbol L1CAM
Gene ID (NCBI) 3897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32004
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

实验方案

Product Specific Protocols
WB protocol for L1CAM antibody 84034-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols