APOB Recombinant antibody
APOB Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
240884H4
验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse liver tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84047-1-RR targets APOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | apolipoprotein B (including Ag(x) antigen) |
Synonyms | Apolipoprotein B, ApoB48, ApoB100, Apo B-100, Apo B |
Calculated Molecular Weight | 516 kDa |
Observed Molecular Weight | 150-250 kDa, 400-520 kDa |
GenBank Accession Number | NM_000384 |
Gene Symbol | APOB |
Gene ID (NCBI) | 338 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P04114 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for APOB antibody 84047-1-RR | Download protocol |
IHC protocol for APOB antibody 84047-1-RR | Download protocol |
IF protocol for APOB antibody 84047-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |