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验证数据展示

  • WB analysis using 84058-4-RR

    WB analysis using 84058-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-4-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84058-4-RR

    WB analysis using 84058-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-4-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 84058-4-RR

    IHC staining of mouse testis using 84058-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84058-4-RR (RNF40 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 84058-4-RR

    IHC staining of mouse testis using 84058-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84058-4-RR (RNF40 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84058-4-RR

    Affinity and Kinetic Characterization of 84058-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84058-4-RR against Human RNF40 were performed. The affinity constant is 1.34 nM.

RNF40 Recombinant antibody

RNF40 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84058-4-RR

产品说明书

CloneNo. 241214D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, A375 cells, HEK-293 cells, HepG2 cells, MCF-7 cells, mouse liver tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84058-4-RR targets RNF40 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RNF40 fusion protein Ag8047 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 40
别名 E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein
计算分子量 114 kDa
观测分子量130-140 kDa
GenBank蛋白编号BC006133
基因名称 RNF40
Gene ID (NCBI) 9810
RRIDAB_3671625
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO75150
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].

实验方案

Product Specific Protocols
WB protocol for RNF40 antibody 84058-4-RRDownload protocol
IHC protocol for RNF40 antibody 84058-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols