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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-4-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-4-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84058-4-RR (RNF40 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84058-4-RR (RNF40 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84058-4-RR

    Affinity and Kinetic Characterization of 84058-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84058-4-RR against Human RNF40 were performed. The affinity constant is 1.34 nM.

RNF40 Recombinant antibody, PBS Only

RNF40 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84058-4-PBS

Print datasheet

CloneNo. 241214D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

84058-4-PBS targets RNF40 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen RNF40 fusion protein Ag8047 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ring finger protein 40
Synonyms E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein
Calculated Molecular Weight 114 kDa
Observed Molecular Weight130-140 kDa
GenBank Accession NumberBC006133
Gene Symbol RNF40
Gene ID (NCBI) 9810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75150
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].