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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
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0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-4-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-4-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84058-4-RR (RNF40 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84058-4-RR (RNF40 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84058-4-RR

    Affinity and Kinetic Characterization of 84058-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84058-4-RR against Human RNF40 were performed. The affinity constant is 1.34 nM.

RNF40 Recombinant antibody, PBS Only

RNF40 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84058-4-PBS

产品说明书

CloneNo. 241214D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84058-4-PBS targets RNF40 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 RNF40 fusion protein Ag8047 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 40
别名 E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein
计算分子量 114 kDa
观测分子量130-140 kDa
GenBank蛋白编号BC006133
基因名称 RNF40
Gene ID (NCBI) 9810
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO75150
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].