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验证数据展示

  • WB analysis using 84083-2-RR

    WB analysis using 84083-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84083-2-RR (VGLUT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 84083-2-RR

    IF Staining of mouse brain using 84083-2-RR

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using VGLUT2 antibody (84083-2-RR, Clone: 241255B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84083-2-RR

    Affinity and Kinetic Characterization of 84083-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84083-2-RR against Human VGLUT2 were performed. The affinity constant is 1.48 nM.

VGLUT2 Recombinant antibody

VGLUT2 Uni-rAbTM Recombinant Antibody for WB, IF-Fro, ELISA
Cat No. 84083-2-RR

产品说明书

CloneNo. 241255B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-Fro, ELISA

别名

SLC17A6, 241255B5, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inunboiled mouse brain tissue
Positive IF-Fro detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84083-2-RR targets VGLUT2 in WB, IF-Fro, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-Fro, ELISA Application Description
经测试反应性 human, mouse
免疫原 VGLUT2 fusion protein Ag29565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
别名 SLC17A6, 241255B5, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI
计算分子量 64 kDa
观测分子量60 kDa
GenBank蛋白编号NM_020346
基因名称 VGLUT2
Gene ID (NCBI) 57084
RRIDAB_3671651
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P2U8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicles (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).

实验方案

Product Specific Protocols
WB protocol for VGLUT2 antibody 84083-2-RRDownload protocol
IF protocol for VGLUT2 antibody 84083-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols