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VGLUT2 Recombinant antibody, PBS Only (Detector)

VGLUT2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241255B5

Cat no : 84083-2-PBS

Print datasheet

Synonyms

SLC17A6, DNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI, 241255B5

验证数据展示

  • WB analysis of mouse brain using 84083-2-RR (same clone as 84083-2-PBS)

    WB analysis of mouse brain using 84083-2-RR (same clone as 84083-2-PBS)

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 84083-2-RR (VGLUT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84083-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00998-1

    Cytometric bead array standard curve of MP00998-1

    Cytometric bead array standard curve of MP00998-1, SLC17A6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84083-1-PBS. Detection antibody: 84083-2-PBS. Standard: Ag29565. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84083-2-RR

    Affinity and Kinetic Characterization of 84083-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84083-2-RR against Human VGLUT2 were performed. The affinity constant is 1.48 nM.

产品信息

84083-2-PBS targets VGLUT2 as part of a matched antibody pair:

MP00998-1: 84083-1-PBS capture and 84083-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen VGLUT2 fusion protein Ag29565 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms SLC17A6, DNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI, 241255B5
Calculated Molecular Weight 64 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberNM_020346
Gene Symbol VGLUT2
Gene ID (NCBI) 57084
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P2U8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicles (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).