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Mouse IGFBP2 Recombinant antibody, PBS Only (Capture)

IGFBP2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241363D12

Cat no : 84092-1-PBS

Print datasheet

Synonyms

241363D12

验证数据展示

  • WB analysis using 84092-1-RR (same clone as 84092-1-PBS)

    WB analysis using 84092-1-RR (same clone as 84092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84092-1-RR (IGFBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84092-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01011-1

    Sandwich ELISA standard curve of MP01011-1

    Sandwich ELISA standard curve of MP01011-1, Mouse IGFBP2 Recombinant Matched Antibody Pair - PBS only. 84092-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1196. 84092-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP01011-1

    Sample test of MP01011-1

    One mouse kidney was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 10 μg/mL of PHA and 10 ng/mL of PMA. An aliquot of the cell culture supernatant was removed, assayed for mouse IGFBP2 and measured 38.18 ng/mL.

  • Sample test of MP01011-1

    Sample test of MP01011-1

    Serum of six mouse was measured. The IGFBP2 concentration of detected samples was determined to be 152.26 ng/mL with a range of 95.84-279.86 ng/mL.

  • Cytometric bead array standard curve of MP01011-1

    Cytometric bead array standard curve of MP01011-1

    Cytometric bead array standard curve of MP01011-1, MOUSE IGFBP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84092-1-PBS. Detection antibody: 84092-2-PBS. Standard: Eg1196. Range: 1.563-100 ng/mL.

产品信息

84092-1-PBS targets IGFBP2 as part of a matched antibody pair:

MP01011-1: 84092-1-PBS capture and 84092-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin-like growth factor binding protein 2
Synonyms 241363D12
Calculated Molecular Weight33kDa
Observed Molecular Weight33-35 kDa
GenBank Accession NumberNM_008342.3
Gene Symbol Igfbp2
Gene ID (NCBI) 16008
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47877
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGFBP2 is a key member of the IGFBP family that is highly expressed in embryonic and fetal tissues and decreases significantly after birth. IGFBP2 mediates IGF-independent tumorigenesis by participating in intracellular and nuclear regulatory networks and may serve as an excellent prognostic biomarker in various cancers (PMID: 35546443).