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Complement factor B Recombinant antibody

Complement factor B Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241141A8

Cat no : 84101-5-RR

Print datasheet

Synonyms

CFB, CFAB, C3/C5 convertase, BFD, BF

验证数据展示

  • WB analysis using 84101-5-RR

    WB analysis using 84101-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84101-5-RR (Complement factor B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84101-5-RR

    IHC staining of mouse liver using 84101-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 84101-5-RR

    IHC staining of mouse liver using 84101-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 84101-5-RR

    IF Staining of HepG2 using 84101-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor B antibody (84101-5-RR, Clone: 241141A8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inmouse serum, rat serum, rat plasma, human plasma
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84101-5-RR is Complement factor B Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Complement factor B fusion protein Ag0226 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name complement factor B
Synonyms CFB, CFAB, C3/C5 convertase, BFD, BF
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 93-100 kDa
GenBank Accession NumberBC007990
Gene Symbol Complement factor B
Gene ID (NCBI) 629
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP00751
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease that is associated with C3b and forms the alternative pathway for C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

实验方案

Product Specific Protocols
WB protocol for Complement factor B antibody 84101-5-RRDownload protocol
IHC protocol for Complement factor B antibody 84101-5-RRDownload protocol
IF protocol for Complement factor B antibody 84101-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols