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0
Conjugate
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验证数据展示

  • WB analysis using 84101-5-RR (same clone as 84101-5-PBS)

    WB analysis using 84101-5-RR (same clone as 84101-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84101-5-RR (Complement factor B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84101-5-RR (same clone as 84101-5-PBS)

    IHC staining of mouse liver using 84101-5-RR (same clone as 84101-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84101-5-RR (same clone as 84101-5-PBS)

    IHC staining of mouse liver using 84101-5-RR (same clone as 84101-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84101-5-RR (same clone as 84101-5-PBS)

    IF Staining of HepG2 using 84101-5-RR (same clone as 84101-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor B antibody (84101-5-RR, Clone: 241141A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84101-5-RR

    Affinity and Kinetic Characterization of 84101-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84101-5-RR against Human Complement factor B were performed. The affinity constant is 2.27 nM.

Complement factor B Recombinant antibody, PBS Only

Complement factor B Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84101-5-PBS

产品说明书

CloneNo. 241141A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CFB, CFAB, C3/C5 convertase, BFD, BF

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84101-5-PBS targets Complement factor B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Complement factor B fusion protein Ag0226 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement factor B
别名 CFB, CFAB, C3/C5 convertase, BFD, BF
计算分子量 86 kDa
观测分子量 93-100 kDa
GenBank蛋白编号BC007990
基因名称 Complement factor B
Gene ID (NCBI) 629
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00751
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease that is associated with C3b and forms the alternative pathway for C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.