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验证数据展示

  • WB analysis using 84127-2-RR

    WB analysis using 84127-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84127-2-RR (DPP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of pig brain using 84127-2-RR

    IP experiment of pig brain using 84127-2-RR

    IP result of anti-DPP10 (IP:84127-2-RR, 4ug; Detection:84127-2-RR 1:1000) with pig brain tissue lysate 1240 ug.

  • Affinity and Kinetic Characterization of 84127-2-RR

    Affinity and Kinetic Characterization of 84127-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84127-2-RR against Human DPP10 were performed. The affinity constant is 1.32 nM.

DPP10 Recombinant monoclonal antibody

DPP10 Uni-rAb® Recombinant Antibody for WB, IP, ELISA
Cat No. 84127-2-RR

产品说明书

CloneNo. 241446A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IP, ELISA

别名

DPL2, Dipeptidyl peptidase-like protein 2, Dipeptidyl peptidase X, Dipeptidyl peptidase IV-related protein 3, dipeptidyl peptidase 10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inpig brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84127-2-RR targets DPP10 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag3231

Product name: Recombinant human DPP10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 448-796 aa of BC030832

Sequence: RGRQLYSASTEGLLNRQCISCNFMKEQCTYFDASFSPMNQHFLLFCEGPRVPVVSLHSTDNPAKYFILESNSMLKEAILKKKIGKPEIKILHIDDYELPLQLSLPKDFMDRNQYALLLIMDEEPGGQLVTDKFHIDWDSVLIDMDNVIVARFDGRGSGFQGLKILQEIHRRLGSVEVKDQITAVKFLLKLPYIDSKRLSIFGKGYGGYIASMILKSDEKLFKCGSVVAPITDLKLYASAFSERYLGMPSKEESTYQAASVLHNVHGLKEENILIIHGTADTKVHFQHSAELIKHLIKAGVNYTMQVYPDEGHNVSEKSKYHLYSTILKFFSDCLKEEISVLPQEPEEDE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dipeptidyl-peptidase 10
别名 DPL2, Dipeptidyl peptidase-like protein 2, Dipeptidyl peptidase X, Dipeptidyl peptidase IV-related protein 3, dipeptidyl peptidase 10
计算分子量 796 aa, 91 kDa
观测分子量91 kDa
GenBank蛋白编号BC030832
基因名称 DPP10
Gene ID (NCBI) 57628
RRIDAB_3671686
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8N608
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPP10 is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. DPP10 is an inactive peptidase that modulates the electrophysiological properties, cell-surface expression and subcellular localization of voltage-gated potassium channels.

实验方案

Product Specific Protocols
IP protocol for DPP10 antibody 84127-2-RRDownload protocol
WB protocol for DPP10 antibody 84127-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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