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验证数据展示

  • WB analysis using 84127-2-RR (same clone as 84127-2-PBS)

    WB analysis using 84127-2-RR (same clone as 84127-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84127-2-RR (DPP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84127-2-PBS in a different storage buffer formulation.

  • IP experiment of pig brain using 84127-2-RR (same clone as 84127-2-PBS)

    IP experiment of pig brain using 84127-2-RR (same clone as 84127-2-PBS)

    IP result of anti-DPP10 (IP:84127-2-RR, 4ug; Detection:84127-2-RR 1:1000) with pig brain tissue lysate 1240 ug. This data was developed using the same antibody clone with 84127-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01035-3

    Sandwich ELISA standard curve of MP01035-3

    Sandwich ELISA standard curve of MP01035-3, Human DPP10 Recombinant Matched Antibody Pair - PBS only. 84127-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3231. 84127-3-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 ng/mL

  • Sample test of MP01035-3

    Sample test of MP01035-3

    HT-29 cell lysate was measured. The human DPP10 concentration of detected samples was determined to be 3,448.25 ng/mL (based on a 4.4 mg/mL extract load).

  • Cytometric bead array standard curve of MP01035-1

    Cytometric bead array standard curve of MP01035-1

    Cytometric bead array standard curve of MP01035-1, DPP10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84127-3-PBS. Detection antibody: 84127-2-PBS. Standard: Ag3231. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 84127-2-RR

    Affinity and Kinetic Characterization of 84127-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84127-2-RR against Human DPP10 were performed. The affinity constant is 1.32 nM.

DPP10 Recombinant antibody, PBS Only (Capture/Detector)

DPP10 Uni-rAbTM Recombinant Antibody for WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84127-2-PBS

产品说明书

CloneNo. 241446A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

DPL2, Dipeptidyl peptidase-like protein 2, Dipeptidyl peptidase X, Dipeptidyl peptidase IV-related protein 3, dipeptidyl peptidase 10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84127-2-PBS targets DPP10 as part of a matched antibody pair:

MP01035-1: 84127-3-PBS capture and 84127-2-PBS detection (validated in Cytometric bead array)

MP01035-3: 84127-2-PBS capture and 84127-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat, pig
免疫原 DPP10 fusion protein Ag3231 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dipeptidyl-peptidase 10
别名 DPL2, Dipeptidyl peptidase-like protein 2, Dipeptidyl peptidase X, Dipeptidyl peptidase IV-related protein 3, dipeptidyl peptidase 10
计算分子量 796 aa, 91 kDa
观测分子量91 kDa
GenBank蛋白编号BC030832
基因名称 DPP10
Gene ID (NCBI) 57628
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N608
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DPP10 is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. DPP10 is an inactive peptidase that modulates the electrophysiological properties, cell-surface expression and subcellular localization of voltage-gated potassium channels.