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DPP10 Recombinant antibody

DPP10 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

241446A5

Cat no : 84127-2-RR

Print datasheet

Synonyms

DPL2, Dipeptidyl peptidase-like protein 2, Dipeptidyl peptidase X, Dipeptidyl peptidase IV-related protein 3, dipeptidyl peptidase 10

验证数据展示

  • WB analysis using 84127-2-RR

    WB analysis using 84127-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84127-2-RR (DPP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of pig brain using 84127-2-RR

    IP experiment of pig brain using 84127-2-RR

    IP result of anti-DPP10 (IP:84127-2-RR, 4ug; Detection:84127-2-RR 1:1000) with pig brain tissue lysate 1240 ug.

  • Affinity and Kinetic Characterization of 84127-2-RR

    Affinity and Kinetic Characterization of 84127-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84127-2-RR against Human DPP10 were performed. The affinity constant is 1.32 nM.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inpig brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84127-2-RR targets DPP10 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen DPP10 fusion protein Ag3231 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dipeptidyl-peptidase 10
Synonyms DPL2, Dipeptidyl peptidase-like protein 2, Dipeptidyl peptidase X, Dipeptidyl peptidase IV-related protein 3, dipeptidyl peptidase 10
Calculated Molecular Weight 796 aa, 91 kDa
Observed Molecular Weight91 kDa
GenBank Accession NumberBC030832
Gene Symbol DPP10
Gene ID (NCBI) 57628
RRIDAB_3671686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N608
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPP10 is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. DPP10 is an inactive peptidase that modulates the electrophysiological properties, cell-surface expression and subcellular localization of voltage-gated potassium channels.

实验方案

Product Specific Protocols
WB protocol for DPP10 antibody 84127-2-RRDownload protocol
IP protocol for DPP10 antibody 84127-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols