• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Phospho-JUN (Ser63) Recombinant antibody

Phospho-JUN (Ser63) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240965B7

Cat no : 84147-1-RR

Print datasheet

Synonyms

AP1, AP 1, JUN, c Jun, Activator protein 1

验证数据展示

  • WB analysis using 84147-1-RR

    WB analysis using 84147-1-RR

    Non-treated NIH/3T3 cells, Anisomycin treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84147-1-RR (Phospho-JUN (Ser63) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

经过测试的应用

Positive WB detected inAnisomycin treated NIH/3T3 cells, UV treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy JUN Ready-To-Use IHC Kit. JUN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84147-1-RR targets Phospho-JUN (Ser63) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name jun oncogene
Synonyms AP1, AP 1, JUN, c Jun, Activator protein 1
Calculated Molecular Weight 331 aa, 36 kDa
Observed Molecular Weight40-48 kDa
GenBank Accession NumberBC068522
Gene Symbol JUN
Gene ID (NCBI) 3725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05412
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)

实验方案

Product Specific Protocols
WB protocol for Phospho-JUN (Ser63) antibody 84147-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols