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Kininogen-1 Recombinant antibody

Kininogen-1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241397C11

Cat no : 84175-5-RR

Print datasheet

Synonyms

KNG1, HMWK, Fitzgerald factor, Bradykinin, BDK

验证数据展示

  • WB analysis of rat plasma using 84175-5-RR

    WB analysis of rat plasma using 84175-5-RR

    Rat plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human plasma using 84175-5-RR

    WB analysis of human plasma using 84175-5-RR

    Human plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HT-29 using 84175-5-RR

    FC experiment of HT-29 using 84175-5-RR

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Kininogen-1 Recombinant antibody (84175-5-RR, Clone:241397C11) (red), or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84175-5-RR

    Affinity and Kinetic Characterization of 84175-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84175-5-RR against Human Kininogen-1 were performed. The affinity constant is 0.182 nM.

经过测试的应用

Positive WB detected inrat plasma, human plasma

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84175-5-RR is Kininogen-1 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kininogen 1
Synonyms KNG1, HMWK, Fitzgerald factor, Bradykinin, BDK
Calculated Molecular Weight72kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberNM_001102416.3
Gene Symbol Kininogen 1
Gene ID (NCBI) 3827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01042-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

实验方案

Product Specific Protocols
WB protocol for Kininogen-1 antibody 84175-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols