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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human plasma using 84175-5-RR (same clone as 84175-5-PBS)

    WB analysis of human plasma using 84175-5-RR (same clone as 84175-5-PBS)

    Human plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84175-5-PBS in a different storage buffer formulation.

  • FC experiment of HT-29 using 84175-5-RR (same clone as 84175-5-PBS)

    FC experiment of HT-29 using 84175-5-RR (same clone as 84175-5-PBS)

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Kininogen-1 Recombinant antibody (84175-5-RR, Clone:241397C11) (red), or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84175-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84175-5-RR

    Affinity and Kinetic Characterization of 84175-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84175-5-RR against Human Kininogen-1 were performed. The affinity constant is 0.182 nM.

Kininogen 1 Recombinant antibody, PBS Only

Kininogen 1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84175-5-PBS

产品说明书

CloneNo. 241397C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

KNG1, 241397C11, Alpha-2-thiol proteinase inhibitor, BDK, Bradykinin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84175-5-PBS targets Kininogen 1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kininogen 1
别名 KNG1, 241397C11, Alpha-2-thiol proteinase inhibitor, BDK, Bradykinin
计算分子量72kDa
观测分子量 48 kDa
GenBank蛋白编号NM_001102416.3
基因名称 Kininogen 1
Gene ID (NCBI) 3827
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01042-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.