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验证数据展示

  • IF Staining of A431 using 84184-5-RR

    IF Staining of A431 using 84184-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MED6 antibody (84184-5-RR, Clone: 241382B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84184-5-RR

    Affinity and Kinetic Characterization of 84184-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84184-5-RR against Human MED6 were performed. The affinity constant is below 1 pM.

MED6 Recombinant antibody

MED6 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 84184-5-RR

产品说明书

CloneNo. 241382B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84184-5-RR targets MED6 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MED6 fusion protein Ag7541 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mediator complex subunit 6
别名 NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component
计算分子量 28 kDa
GenBank蛋白编号BC004106
基因名称 MED6
Gene ID (NCBI) 10001
RRIDAB_3671739
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO75586
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

实验方案

Product Specific Protocols
IF protocol for MED6 antibody 84184-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols