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验证数据展示

  • IF Staining of A431 using 84184-5-RR (same clone as 84184-5-PBS)

    IF Staining of A431 using 84184-5-RR (same clone as 84184-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MED6 antibody (84184-5-RR, Clone: 241382B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84184-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84184-5-RR

    Affinity and Kinetic Characterization of 84184-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84184-5-RR against Human MED6 were performed. The affinity constant is below 1 pM.

MED6 Recombinant antibody, PBS Only

MED6 Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 84184-5-PBS

产品说明书

CloneNo. 241382B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Indirect ELISA

别名

NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84184-5-PBS targets MED6 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 MED6 fusion protein Ag7541 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mediator complex subunit 6
别名 NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component
计算分子量 28 kDa
GenBank蛋白编号BC004106
基因名称 MED6
Gene ID (NCBI) 10001
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO75586
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.