• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84231-4-RR

    WB analysis using 84231-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84231-4-RR (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 84231-4-RR

    IF Staining of K-562 using 84231-4-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (84231-4-RR, Clone: 241518D9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84231-4-RR

    Affinity and Kinetic Characterization of 84231-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84231-4-RR against Human TNIK were performed. The affinity constant is 2.01 nM.

TNIK Recombinant antibody

TNIK Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84231-4-RR

产品说明书

CloneNo. 241518D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1, 241518D9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inT-47D cells, HeLa cells, HEK-293 cells, K-562 cells, COLO 205 cells, Caco-2 cells
Positive IF/ICC detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84231-4-RR targets TNIK in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TNIK fusion protein Ag34132 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TRAF2 and NCK interacting kinase
别名 TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1, 241518D9
观测分子量170 kDa
GenBank蛋白编号BC150256
基因名称 TNIK
Gene ID (NCBI) 23043
RRIDAB_3671785
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UKE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).

实验方案

Product Specific Protocols
WB protocol for TNIK antibody 84231-4-RRDownload protocol
IF protocol for TNIK antibody 84231-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols