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TNIK Recombinant antibody, PBS Only

TNIK Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241518D9

Cat no : 84231-4-PBS

Print datasheet

Synonyms

TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1, 241518D9

验证数据展示

  • WB analysis using 84231-4-RR (same clone as 84231-4-PBS)

    WB analysis using 84231-4-RR (same clone as 84231-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84231-4-RR (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84231-4-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 84231-4-RR (same clone as 84231-4-PBS)

    IF Staining of K-562 using 84231-4-RR (same clone as 84231-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (84231-4-RR, Clone: 241518D9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84231-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84231-4-RR

    Affinity and Kinetic Characterization of 84231-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84231-4-RR against Human TNIK were performed. The affinity constant is 2.01 nM.

产品信息

The immunogen of 84231-4-PBS is TNIK Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TNIK fusion protein Ag34132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TRAF2 and NCK interacting kinase
Synonyms TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1, 241518D9
Observed Molecular Weight170 kDa
GenBank Accession NumberBC150256
Gene Symbol TNIK
Gene ID (NCBI) 23043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UKE5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).