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TNIK Recombinant antibody

TNIK Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241518D9

Cat no : 84231-4-RR

Print datasheet

Synonyms

TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1, 241518D9

验证数据展示

  • WB analysis using 84231-4-RR

    WB analysis using 84231-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84231-4-RR (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 84231-4-RR

    IF Staining of K-562 using 84231-4-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (84231-4-RR, Clone: 241518D9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84231-4-RR

    Affinity and Kinetic Characterization of 84231-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84231-4-RR against Human TNIK were performed. The affinity constant is 2.01 nM.

经过测试的应用

Positive WB detected inT-47D cells, HeLa cells, HEK-293 cells, K-562 cells, COLO 205 cells, Caco-2 cells
Positive IF/ICC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84231-4-RR is TNIK Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TNIK fusion protein Ag34132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TRAF2 and NCK interacting kinase
Synonyms TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1, 241518D9
Observed Molecular Weight170 kDa
GenBank Accession NumberBC150256
Gene Symbol TNIK
Gene ID (NCBI) 23043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UKE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).

实验方案

Product Specific Protocols
WB protocol for TNIK antibody 84231-4-RRDownload protocol
IF protocol for TNIK antibody 84231-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols